Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

Quantification of adipocyte numbers in transgenic mice via the Cre-LoxP recombination sites

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Summary: This protocol describes a method to assess adipocyte numbers within a specific depot based on their inducible genomic label.By extracting DNA from a complete adipose tissue depot skylight quilt pattern stemming from two transgenic mouse lines (Adipoq-CreERT2 x ROSA26-tdRFP and Ucp1-CreERT2 x ROSA26-tdRFP), the number of adipocytes can be determined based on the quantification of the recombined LoxPRed sites.This highly sensitive system allows for the quantification of white, brown, and brite/beige adipocytes in a spatially unbiased red label violin strings color code and size-independent manner.For complete details on the use and execution of this protocol, please refer to Moser et al.

(2021).